The Scientific Method Research Paper

Mr.kousen is Water Man

WRITING A RESEARCH PAPER USING THE SCIENTIFIC METHOD

When wrtiing a research paper for this Biology Class, there are a series of rules that will help you when you prepare your paper to turn in for an assignment.

1.    Always use the correct steps of the Scientific Method in the proper order.  They are:  Stating the
      Problem, Hypothesis, Experimental Design, Experiment or Test the Hypothesis, Collection of Data,
      Analyzing the Data, and then your Conclusion.
2.    All research papers are major assignments.  They must be completed in black or blue ink if
      Handwritten, typewritten, or computer generated.  Any drawings or illustrations can be completed neatly
      in pencil.
3.    Never, ever, write on the backs of any lab work, drawings, or paper that you are turning in for a
      grade.
4.    If using a computer and printer, font size of any paper should be between 12-14.

5.    Examples of previous research papers, projects, and computer projects will be made available for your
      review as you start a project.  I would suggest you look at the good and bad points of each example
      paper.

STEPS

    PROBLEM-  In the problem you need to state what it is you are trying to solve in your research.  This should be written in the form of a question.  It should also explain the purpose of doing this form of research paper.

    HYPOTHESIS-  This should be written before you do any of your experiment..day one...this is to explain what you believe will explain the answer to your proposed question which you stated earlier.

    EXPERIMENTAL DESIGN-  In this section it is important that you explain everything about what you did to set up the research experiment that you are starting.  This will include background information about where you obtained samples, what the area looked like, dates and times, and exact locations of samples.

    EXPERIMENT OR TEST HYPOTHESIS-  In this section you will explain what you did from beginning to end on a daily basis of how you ran your experiment to test your hypothesis.  You need to explain everything you did, equipment you used, and how you went going about setting up your research.

    COLLECTION OF DATA-    This is where you explain on a daily basis what you observed, changes that took place, drawings, charts, data tables, times, etc.  Only observable information belongs in this section.  No thinking please!  Obnserve and record.  Date all observations correctly.  If you make drawings, they belong in this section along with proper labels and magnifications if you are using a microscope.

    ANALYSIS OF DATA-      This is the section that you can now think!  Try to review your recorded data and see where some relationships may occur to events recorded and the date that it happened.  Now is the time to explain why certain things happened and why you will be coming up with the conclusion that you will.  In other words, use data here to help support the reasons you are coming up with the conclusions to your reesearch.  This section should be the longest along with the data section.

    CONCLUSION-    Last part of the paper.  Here is where you will give your conclusion as to whether your hypothesis has been supported, not supported, or contridicted!  Supported means that your data indicates that your hypothesis was very close to being true as your predicted.  Not supported means that there wasn't enough good data, problems with your experiment, or something went wrong which would not provide you with a way to evaluate your hypothesis.  Contridicted means that the data that you obtained indicates that your hypothesis was incorrect and not close at all to what you predicted.  One of the three underlined words is how you will end your conclusion.  You also need to give a brief paragraph afterwards which indicates why you came up with the conclusion that you did.

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WRITING A SCIENTIFIC RESEARCH ARTICLE

| Format for the paper | Edit your paper! | Useful books |

FORMAT FOR THE PAPER

Scientific research articles provide a method for scientists to communicate with other scientists about the results of their research. A standard format is used for these articles, in which the author presents the research in an orderly, logical manner. This doesn't necessarily reflect the order in which you did or thought about the work.  This format is:

| Title | Authors | Introduction | Materials and Methods | Results (with Tables and Figures) | Discussion | Acknowledgments | Literature Cited |

TITLE

  1. Make your title specific enough to describe the contents of the paper, but not so technical that only specialists will understand. The title should be appropriate for the intended audience.
  2. The title usually describes the subject matter of the article: Effect of Smoking on Academic Performance"
  3. Sometimes a title that summarizes the results is more effective: Students Who Smoke Get Lower Grades"

AUTHORS

1. The person who did the work and wrote the paper is generally listed as the first author of a research paper.

2. For published articles, other people who made substantial contributions to the work are also listed as authors. Ask your mentor's permission before including his/her name as co-author.

ABSTRACT

1. An abstract, or summary, is published together with a research article, giving the reader a "preview" of what's to come. Such abstracts may also be published separately in bibliographical sources, such as Biologic al Abstracts. They allow other scientists to quickly scan the large scientific literature, and decide which articles they want to read in depth. The abstract should be a little less technical than the article itself; you don't want to dissuade your potent ial audience from reading your paper.

2. Your abstract should be one paragraph, of 100-250 words, which summarizes the purpose, methods, results and conclusions of the paper.

3. It is not easy to include all this information in just a few words. Start by writing a summary that includes whatever you think is important, and then gradually prune it down to size by removing unnecessary words, while still retaini ng the necessary concepts.

3. Don't use abbreviations or citations in the abstract. It should be able to stand alone without any footnotes.

INTRODUCTION

What question did you ask in your experiment? Why is it interesting? The introduction summarizes the relevant literature so that the reader will understand why you were interested in the question you asked. One to fo ur paragraphs should be enough. End with a sentence explaining the specific question you asked in this experiment.

MATERIALS AND METHODS

1. How did you answer this question? There should be enough information here to allow another scientist to repeat your experiment. Look at other papers that have been published in your field to get some idea of what is included in this section.

2. If you had a complicated protocol, it may helpful to include a diagram, table or flowchart to explain the methods you used.

3. Do not put results in this section. You may, however, include preliminary results that were used to design the main experiment that you are reporting on. ("In a preliminary study, I observed the owls for one week, and found that 73 % of their locomotor activity occurred during the night, and so I conducted all subsequent experiments between 11 pm and 6 am.")

4. Mention relevant ethical considerations. If you used human subjects, did they consent to participate. If you used animals, what measures did you take to minimize pain?

RESULTS

1. This is where you present the results you've gotten. Use graphs and tables if appropriate, but also summarize your main findings in the text. Do NOT discuss the results or speculate as to why something happened; t hat goes in th e Discussion.

2. You don't necessarily have to include all the data you've gotten during the semester. This isn't a diary.

3. Use appropriate methods of showing data. Don't try to manipulate the data to make it look like you did more than you actually did.

"The drug cured 1/3 of the infected mice, another 1/3 were not affected, and the third mouse got away."

TABLES AND GRAPHS

1. If you present your data in a table or graph, include a title describing what's in the table ("Enzyme activity at various temperatures", not "My results".) For graphs, you should also label the x and y axes.

2. Don't use a table or graph just to be "fancy". If you can summarize the information in one sentence, then a table or graph is not necessary.

DISCUSSION

1. Highlight the most significant results, but don't just repeat what you've written in the Results section. How do these results relate to the original question? Do the data support your hypothesis? Are your results consistent with what other investigators have reported? If your results were unexpected, try to explain why. Is there another way to interpret your results? What further research would be necessary to answer the questions raised by your results? How do y our results fit into the big picture?

2. End with a one-sentence summary of your conclusion, emphasizing why it is relevant.

ACKNOWLEDGMENTS

This section is optional. You can thank those who either helped with the experiments, or made other important contributions, such as discussing the protocol, commenting on the manuscript, or buying you pizza.

REFERENCES (LITERATURE CITED)

There are several possible ways to organize this section. Here is one commonly used way:

1. In the text, cite the literature in the appropriate places:

Scarlet (1990) thought that the gene was present only in yeast, but it has since been identified in the platypus (Indigo and Mauve, 1994) and wombat (Magenta, et al., 1995).

2. In the References section list citations in alphabetical order.

Indigo, A. C., and Mauve, B. E. 1994. Queer place for qwerty: gene isolation from the platypus. Science 275, 1213-1214.

Magenta, S. T., Sepia, X., and Turquoise, U. 1995. Wombat genetics. In: Widiculous Wombats, Violet, Q., ed. New York: Columbia University Press. p 123-145.

Scarlet, S.L. 1990. Isolation of qwerty gene from S. cerevisae. Journal of Unusual Results 36, 26-31.

 

EDIT YOUR PAPER!!!

"In my writing, I average about ten pages a day. Unfortunately, they're all the same page."

A major part of any writing assignment consists of re-writing.

Write accurately

  1. Scientific writing must be accurate. Although writing instructors may tell you not to use the same word twice in a sentence, it's okay for scientific writing, which must be accurate. (A student who tried not to repeat the word "hamster" produced this confusing sentence: "When I put the hamster in a cage with the other animals, the little mammals began to play.")
  2. Make sure you say what you mean.
  3. Instead of: The rats were injected with the drug. (sounds like a syringe was filled with drug and ground-up rats and both were injected together)
    Write: I injected the drug into the rat.

  4. Be careful with commonly confused words:

Temperature has an effect on the reaction.
Temperature affects the reaction.

I used solutions in various concentrations. (The solutions were 5 mg/ml, 10 mg/ml, and 15 mg/ml)
I used solutions in varying concentrations. (The concentrations I used changed; sometimes they were 5 mg/ml, other times they were 15 mg/ml.)

 Less food (can't count numbers of food)
Fewer animals (can count numbers of animals)

A large amount of food (can't count them)
A large number of animals (can count them)

The erythrocytes, which are in the blood, contain hemoglobin.
The erythrocytes that are in the blood contain hemoglobin. (Wrong. This sentence implies that there are erythrocytes elsewhere that don't contain hemoglobin.)

Write clearly

1. Write at a level that's appropriate for your audience.

"Like a pigeon, something to admire as long as it isn't over your head." Anonymous

 2. Use the active voice. It's clearer and more concise than the passive voice.

 Instead of: An increased appetite was manifested by the rats and an increase in body weight was measured.
Write: The rats ate more and gained weight.

 3. Use the first person.

 Instead of: It is thought
Write: I think

 Instead of: The samples were analyzed
Write: I analyzed the samples

 4. Avoid dangling participles.

 "After incubating at 30 degrees C, we examined the petri plates." (You must've been pretty warm in there.)

 Write succinctly

 1. Use verbs instead of abstract nouns

 Instead of: take into consideration
Write: consider

 2. Use strong verbs instead of "to be"

 Instead of: The enzyme was found to be the active agent in catalyzing...
Write: The enzyme catalyzed...

 3. Use short words.

"I would never use a long word where a short one would answer the purpose. I know there are professors in this country who 'ligate' arteries. Other surgeons tie them, and it stops the bleeding just as well."
Oliver Wendell Holmes, Sr .

have
sufficientenough
utilizeuse
demonstrateshow
assistancehelp
terminateend

4. Use concise terms.

 Instead of:Write:
prior to before
due to the fact thatbecause
in a considerable number of casesoften
the vast majority ofmost
during the time thatwhen
in close proximity tonear
it has long been known thatI'm too lazy to look up the reference

5. Use short sentences. A sentence made of more than 40 words should probably be rewritten as two sentences.

 "The conjunction 'and' commonly serves to indicate that the writer's mind still functions even when no signs of the phenomenon are noticeable." Rudolf Virchow, 1928

  

Check your grammar, spelling and punctuation

1. Use a spellchecker, but be aware that they don't catch all mistakes.

 "When we consider the animal as a hole,..." Student's paper

 2. Your spellchecker may not recognize scientific terms. For the correct spelling, try Biotech's Life Science Dictionary or one of the technical dictionaries on the reference shelf in the Biology or Health Sciences libraries.

 3. Don't, use, unnecessary, commas.

 4. Proofread carefully to see if you any words out.

USEFUL BOOKS

Victoria E. McMillan, Writing Papers in the Biological Sciences, Bedford Books, Boston, 1997
The best. On sale for about $18 at Labyrinth Books, 112th Street. On reserve in Biology Library

Jan A. Pechenik, A Short Guide to Writing About Biology, Boston: Little, Brown, 1987

Harrison W. Ambrose, III & Katharine Peckham Ambrose, A Handbook of Biological Investigation, 4th edition, Hunter Textbooks Inc, Winston-Salem, 1987
Particularly useful if you need to use statistics to analyze your data. Copy on Reference shelf in Biology Library.

Robert S. Day, How to Write and Publish a Scientific Paper, 4th edition, Oryx Press, Phoenix, 1994.
Earlier editions also good. A bit more advanced, intended for those writing papers for publication. Fun to read. Several copies available in Columbia libraries.

William Strunk, Jr. and E. B. White, The Elements of Style, 3rd ed. Macmillan, New York, 1987.
Several copies available in Columbia libraries.  Strunk's first edition is available on-line.

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